An H+-ATPase regulates cytoplasmic pH in Pneumocystis carinii trophozoites

Why have Pneumocystis carinii trophozoites been ignored?

Giemsa staining for cysts and trophozoites of Pneumocystis carinii.

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining w...

A vacuolar type H(+)-ATPase regulates cytoplasmic pH in murine macrophages.

An Na(+)- and HCO3(-)-independent mechanism of cytoplasmic pH (pHi) recovery was previously demonstrated in acid-loaded macrophages (Swallow, C. J., Grinstein, S., and Rotstein, O. D. (1988) J. Biol. Chem. 263, 19558-19563). Acid extrusion was found to be ATP-dependent and sensitive to N-ethylmaleimide and N,N'-dicyclohexylcarbodiimide, suggesting involvement of an H(+)-pumping ATPase. In this ...

Growth of Pneumocystis Carinii in Axenic Culture

SUMMARY In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cyclo­heximide, penicillin, & streptomycin to neopeptone­N-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its...

Pneumocystis carinii.

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...